Description
Overview
Lucid Scienti?c’s Resipher empowers researchers to precisely measure oxygen consumption directly in standard well plates. The system’s patented dynamic optical oxygen sensors provide the highest sensitivity without disturbing cells. Resipher’s super- compact pro?le sits directly in your incubator, eliminating the need to change current work?ows and eliminating the need to constantly move plates to larger analytical instruments.
Resipher’s web-based, real-time logging and analysis software provides fast and easy data visualization for each well being monitored. Scientists can now watch their cellular experiments real- time from their computer or remotely via smartphone or tablet.
RESIPHER devices utilize proprietary high-resolution optical oxygen sensors to
characterize oxygen consumption and the oxygen environment in cell culture. The RESIPHER is a handheld device that rests on top of a 96-well plate in the incubator. The device interfaces with a sterile/disposable lid with probes that extend into the media directly above the cells.
Micro probes (500μm diameter) are non-invasive to the cell culture.
An oxygen concentration gradient forms in the media as a direct result of cellular oxygen consumption. The readout is attained by dynamically scanning above the cells to measure the gradient, then using sophisticated signal processing algorithms to convert concentration readings to cellular oxygen consumption.
Resipher streams real-time data to a web-based analysis and visualization software by the way of a hub which resides outside the incubator and supports up to 8 devices simultaneously.
In addition to oxygen consumption, the user also has access to a characterization of oxygen concentration, incubator temperature, relative humidity, atmospheric pressure, device motion and several other environmental factors.
Features
Characterize real-time metabolic response with sensitive dynamic probes that minimize error.
Resipher’s compact size occupies no extra incubator space.
Individually sterilized disposable lids attach between the RESIPHER and the well plate.
Resipher is compatible with standard 96 well plates, and currently has 32 well probes per device.
Real-time continuous data analysis
Plug and play USB-C connection
Non-invasive measurements
Scalable sensor con?gurations
Journal Citations
BIOMATERIALS ENGINEERING
Rabussier, G.; Bunter, I.; Bouwhuis, J. et. al. (2023) Healthy and diseased placental barrier on-a-chip models suitable for standardized studies, Acta Biomaterialia.
CELLULAR BEHAVIOR IN HYPOXIA
Smith, M.; Yang, F.; Griffiths, A. et. al. (2023) Redox and metal profiles in human coronary endothelial and smooth muscle cells under hyperoxia, physiological normoxia and hypoxia: Effects of NRF2 signaling on intracellular zinc, Redox Biology, 62, 102712.
LUNAR DUST
Chang, J.H.; Xue, Z.; Bauer, J. et. al. (2023) Artificial Space Weathering to Mimic Solar Wind Enhances the Toxicity of Lunar Dust Simulants in Human Lung Cells, Authorea.
CANCER THERAPY
Mohan, A.; Griffith, K.A.; Wuchu, F. et. al. (2023) Devimistat in combination with gemcitabine and cisplatin in biliary tract cancer: Pre-clinical evaluation and phase 1b multicenter clinical trial (BilT-04), Clin Cancer Res ,CCR-23-0036.
IMMUNOTHERAPY AND CANCER METABOLISM
Bell, H.; Huber, A.; Singhal, R. et. al. (2023) Microenvironmental ammonia enhances T cell exhaustion in colorectal cancer, Cell Metabolism, 35, 134-149.
EFFECTS OF OXYGEN AVAILABILITY ON CELL METABOLISM
Tan, J.; Virtue, S. et al. (2022) Oxygen is a critical regulator of cellular metabolism and function in cell culture. bioRxiv.
BACTERIAL ANTIBIOTIC RESISTANCE
Palomino, A., Gewurz, D., DeVine, L. et al. (2023) Metabolic genes on conjugative plasmids are highly prevalent in Escherichia coli and can protect against antibiotic treatment. ISME J. 17, 151
BIOPRINTED 3D MICROALGAE CONSTRUCTS
Dani, S., Windisch, J., XM, V. G., Bernhardt, A., Gelinsky, M., Krujatz, F., & Lode, A. (2022). Selection of a suitable photosynthetically active microalgae strain for the co-cultivation with mammalian cells. Frontiers in Bioengineering and Biotechnology, 10, 994134.
CANCER METABOLIC PATHWAY
Achreja, A., Yu, T., Mittal, A. et al. (2022). Metabolic collateral lethal target identification reveals MTHFD2 paralogue dependency in ovarian cancer. Nature Metabolism, 4, 1119–1137.
MITOCHONDRIAL METABOLISM IN HYPOXIC CONDITIONS
Salaroglio, I. C., Belisario, D. C., Akman, M., La Vecchia, S., Godel, M., Anobile, D. P., Ortone, G., Digiovanni, S., Fontana, S., Costamagna, C., Rubinstein, M., Kopecka, J., & Riganti, C. (2022). Mitochondrial Ros Drive resistance to chemotherapy and immune-killing in hypoxic non-small cell lung cancer. Journal of Experimental & Clinical Cancer Research, 41, 243
MITOCHONDRIAL DYSFUNCTION
Shu, D. Y., Frank, S. I., Fitch, T. C., Karg, M. M., Butcher, E. R., Nnuji-John, E., Kim, L. A., and Saint-Geniez. M. (2022) Dimethyl Fumarate Blocks Tumor Necrosis Factor-Alpha-Driven Inflammation and Metabolic Rewiring in the Retinal Pigment Epithelium. Front. Mol. Neurosci. 15:896786.
MITOCHONDRIAL ELECTRON TRANSPORT
Spinelli, J. B., Rosen, P. C., Sprenger, H. G., Puszynska, A. M., Mann, J. L., Roessler, J. M., Cangelosi, A. L., Henne, A., Condon, K. J., Zhang, T., Kunchok, T., Lewis, C. A., Chandel, N. S., & Sabatini, D. M. (2021). Fumarate is a terminal electron acceptor in the mammalian electron transport chain. Science, 374(6572), 1227–1237.
CARDIOMYOCYTE METABOLOMICS
Abouleisa, R. R., McNally, L., Salama, A. B. M., Hammad, S. K., Ou, Q., Wells, C., Lorkiewicz, P. K., Bolli, R., Mohamed, T. M., & Hill, B. G. (2021). Cell cycle induction in human cardiomyocytes is dependent on biosynthetic pathway activation. Redox Biology, 46, 102094.
CARDIOMYOCYTE METABOLOMICS
McNally, L. A., Altamimi, T. R., Fulghum, K., & Hill, B. G. (2021). Considerations for using isolated cell systems to understand cardiac metabolism and biology. Journal of Molecular and Cellular Cardiology, 153, 26–41.
MITOCHONDRIAL DYSFUNCTION
Mohiuddin, M., Choi, J. J., Lee, N. H., Jeong, H., Anderson, S. E., Han, W. M., Aliya, B., Peykova, T. Z., Verma, S., García, A. J., Aguilar, C. A., & Jang, Y. C. (2020). Transplantation of Muscle Stem Cell Mitochondria Rejuvenates the Bioenergetic Function of Dystrophic Muscle. bioRxiv.
